Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection
Identifieur interne : 000587 ( Main/Exploration ); précédent : 000586; suivant : 000588Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection
Auteurs : Peter Schirmacher [Allemagne] ; Dagmar Schau [Allemagne] ; Hans Peter Dienes [Allemagne]Source :
- Journal of Hepatology [ 0168-8278 ] ; 1995.
Abstract
Background: Transforming growth factor-alpha is an intracellularly processed and secreted polypeptide that induces a proliferative response in epithelial target cells and represents a potential regulatory factor in embryonic development, liver regeneration, and also hepatocarcinogenesis. We have observed focal transforming growth factor-alpha expression in liver tissues with chronic hepatitis B virus infection. Methods: To further elucidate the nature of this focal transforming growth factor-alpha accumulation were have analyzed overall 23 different liver tissues with chronic hepatitis B virus and hepatitis C virus infection as well as normal liver tissues by immunohistology, ELISA, and Western immunoblot with and without immunoprecipitation. Results: By immunohistology transforming growth factor-alpha polypeptides showed focal subcellular accumulation in ground glass hepatocytes, the histological hallmark of chronic hepatitis B virus infection, in co-localization with HBV-preS1 antigen. By ELISA and Western immunoblot increased tissue concentrations of transforming growth factor-alpha were demonstrated in chronically hepatitis B virus-infected liver tissues with ground glass hepatocytes, especially a 15-kD polypeptide, most likely representing an incompletely processed transforming growth factor-alpha polypeptide. transforming growth factor-alpha retention in ground glass hepatocytes is not a general unspecific effect, since it was not observed for several other secretory liver proteins. Accumulated transforming growth factor-alpha in ground glass hepatocytes does not co-localize with Epidermal Growth Factor Receptor expression. Conclusion: Thus evidence is presented that a principally secreted (viral) polypeptide (HBV-preS1) can interfere with the secretion and processing of a second (cellular) protein (transforming growth factor-alpha). Accumulation of transforming growth factor-alpha may result from alteration of the endoplasmic reticulum due to storage of hepatitis B virus surface antigen particles. No evidence was found for transforming growth factor-alpha in ground glass hepatocytes to intracellularly interact with the Epidermal Growth Factor Receptor.
Url:
DOI: 10.1016/S0168-8278(96)80139-5
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000213
- to stream Istex, to step Curation: 000209
- to stream Istex, to step Checkpoint: 000546
- to stream Main, to step Merge: 000590
- to stream Main, to step Curation: 000587
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection</title><author><name sortKey="Schirmacher, Peter" sort="Schirmacher, Peter" uniqKey="Schirmacher P" first="Peter" last="Schirmacher">Peter Schirmacher</name></author><author><name sortKey="Schau, Dagmar" sort="Schau, Dagmar" uniqKey="Schau D" first="Dagmar" last="Schau">Dagmar Schau</name></author><author><name sortKey="Dienes, Hans Peter" sort="Dienes, Hans Peter" uniqKey="Dienes H" first="Hans Peter" last="Dienes">Hans Peter Dienes</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:777AF08BEA73B4FB9CE51B8A3903666CA72E25C5</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1016/S0168-8278(96)80139-5</idno><idno type="url">https://api.istex.fr/document/777AF08BEA73B4FB9CE51B8A3903666CA72E25C5/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000213</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000213</idno><idno type="wicri:Area/Istex/Curation">000209</idno><idno type="wicri:Area/Istex/Checkpoint">000546</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000546</idno><idno type="wicri:doubleKey">0168-8278:1996:Schirmacher P:intracellular:accumulation:of</idno><idno type="wicri:Area/Main/Merge">000590</idno><idno type="wicri:Area/Main/Curation">000587</idno><idno type="wicri:Area/Main/Exploration">000587</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection</title><author><name sortKey="Schirmacher, Peter" sort="Schirmacher, Peter" uniqKey="Schirmacher P" first="Peter" last="Schirmacher">Peter Schirmacher</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, University of Mainz, Mainz</wicri:regionArea><placeName><region type="land" nuts="2">Rhénanie-Palatinat</region><settlement type="city">Mayence</settlement></placeName></affiliation></author><author><name sortKey="Schau, Dagmar" sort="Schau, Dagmar" uniqKey="Schau D" first="Dagmar" last="Schau">Dagmar Schau</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, University of Mainz, Mainz</wicri:regionArea><placeName><region type="land" nuts="2">Rhénanie-Palatinat</region><settlement type="city">Mayence</settlement></placeName></affiliation></author><author><name sortKey="Dienes, Hans Peter" sort="Dienes, Hans Peter" uniqKey="Dienes H" first="Hans Peter" last="Dienes">Hans Peter Dienes</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, University of Mainz, Mainz</wicri:regionArea><placeName><region type="land" nuts="2">Rhénanie-Palatinat</region><settlement type="city">Mayence</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Hepatology</title><title level="j" type="abbrev">JHEPAT</title><idno type="ISSN">0168-8278</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">24</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="547">547</biblScope><biblScope unit="page" to="554">554</biblScope></imprint><idno type="ISSN">0168-8278</idno></series><idno type="istex">777AF08BEA73B4FB9CE51B8A3903666CA72E25C5</idno><idno type="DOI">10.1016/S0168-8278(96)80139-5</idno><idno type="PII">S0168-8278(96)80139-5</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-8278</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Transforming growth factor-alpha is an intracellularly processed and secreted polypeptide that induces a proliferative response in epithelial target cells and represents a potential regulatory factor in embryonic development, liver regeneration, and also hepatocarcinogenesis. We have observed focal transforming growth factor-alpha expression in liver tissues with chronic hepatitis B virus infection. Methods: To further elucidate the nature of this focal transforming growth factor-alpha accumulation were have analyzed overall 23 different liver tissues with chronic hepatitis B virus and hepatitis C virus infection as well as normal liver tissues by immunohistology, ELISA, and Western immunoblot with and without immunoprecipitation. Results: By immunohistology transforming growth factor-alpha polypeptides showed focal subcellular accumulation in ground glass hepatocytes, the histological hallmark of chronic hepatitis B virus infection, in co-localization with HBV-preS1 antigen. By ELISA and Western immunoblot increased tissue concentrations of transforming growth factor-alpha were demonstrated in chronically hepatitis B virus-infected liver tissues with ground glass hepatocytes, especially a 15-kD polypeptide, most likely representing an incompletely processed transforming growth factor-alpha polypeptide. transforming growth factor-alpha retention in ground glass hepatocytes is not a general unspecific effect, since it was not observed for several other secretory liver proteins. Accumulated transforming growth factor-alpha in ground glass hepatocytes does not co-localize with Epidermal Growth Factor Receptor expression. Conclusion: Thus evidence is presented that a principally secreted (viral) polypeptide (HBV-preS1) can interfere with the secretion and processing of a second (cellular) protein (transforming growth factor-alpha). Accumulation of transforming growth factor-alpha may result from alteration of the endoplasmic reticulum due to storage of hepatitis B virus surface antigen particles. No evidence was found for transforming growth factor-alpha in ground glass hepatocytes to intracellularly interact with the Epidermal Growth Factor Receptor.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Rhénanie-Palatinat</li></region><settlement><li>Mayence</li></settlement></list><tree><country name="Allemagne"><region name="Rhénanie-Palatinat"><name sortKey="Schirmacher, Peter" sort="Schirmacher, Peter" uniqKey="Schirmacher P" first="Peter" last="Schirmacher">Peter Schirmacher</name></region><name sortKey="Dienes, Hans Peter" sort="Dienes, Hans Peter" uniqKey="Dienes H" first="Hans Peter" last="Dienes">Hans Peter Dienes</name><name sortKey="Schau, Dagmar" sort="Schau, Dagmar" uniqKey="Schau D" first="Dagmar" last="Schau">Dagmar Schau</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/MagnificatV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000587 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000587 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= MagnificatV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:777AF08BEA73B4FB9CE51B8A3903666CA72E25C5
|texte= Intracellular accumulation of incompletely processed transforming growth factor-alpha polypeptides in ground glass hepatocytes of chronic hepatitis B virus infection
}}
| This area was generated with Dilib version V0.6.31. |  |